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KMID : 0363020050350020299
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Journal of Korean Academy of Periodontology
2005 Volume.35 No. 2 p.299 ~ p.310
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The IL-1 Gene polymorphisms in Korean Children
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윤성식
정현주/김옥수/양규호
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Abstract
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The severe form of chronic periodontitis(CP) has been reported to be strongly associated
with the presence of allele 2 of composite IL-1B(+3954) and IL-1A(+4845) genetic polymorphisms(genotype positive). However, other studies have reported conflicting findings. These
might have resulted from differences in ethnic background and disease entities.
The aim of this study was to determine the distribution of IL-1A(+4845), IL-1B(+3954),
IL-1B(-511), and IL-1 RN(VNTR) genetic polymorphisms in children as a future Korean
population. The study population consisted of 92 children from the Dept. of Pediatric Dentistry, Chonnam National University Hospital. Genomic DNA was obtained from buccal swab.
The IL-1A(+4845), IL-1B(+3954), and IL-1B(-511) genes were genotyped by amplifying the
polymorphic region using multiplex polymerase chain reaction(PCR), followed by restriction
enzyme digestion and gel electrophoresis. IL-1 RN(VNTR) polymorphism were then evaluated by PCR amplification and fragment size analysis in agarose gel.
The allele 2 frequency was 41.3%, 4.3%, 47.8%, and 9.9% for IL-1A(+4845), IL-1B(+3954),
IL-1B(-511), and IL-1 RN respectively. The frequency of genotype with allele 2 carriage for
IL-1A(+4845), IL-1B(+3954), IL-1B(-511), and IL-1 RN was 77.1%, 7.6%, 63.0%, and 15.2%respectively. The allele 2 frequency in IL-1B(+3954) was significantly higher in female than
in male population(p<0.05). The negative association was shown between the presence of
allele 2 in IL-1B(-511) and in IL-1B(+3954), and the carriage rate of IL-1B(+3954) allele 2
tended to lower in IL-1B(-511) allele 2(P=0.056). Only 7.3% of children carried the composite
genotype of IL-1A(+4845) and IL-1B(+3954).
These results suggest that the polymorphism of IL-1B(+3954) and the positive composite
genotype was relatively rare in Korean population.
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KEYWORD
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IL-1 gene polymorphism, composite genotype, Korean
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